Streptococcus pneumoniae (pneumococcus): Overview

Overview

Streptococcus pneumoniae (pneumococcus) is a Gram-positive bacterium that is responsible for the majority of community-acquired pneumonia. It is a commensal organism in the human respiratory tract, meaning that it benefits from the human body, without harming it. However, infection by pneumococcus may be dangerous, causing not only pneumonia, but also bronchitis, otitis media, septicemia, and meningitis.

3D illustration. Streptococcus pneumoniae, or pneumococcus, is a gram-positive bacteria responsible for many types of pneumococcal infections. Image Copyright: ktsdesign / Shutterstock

S. pneumoniae is alpha-hemolytic, meaning that it can break down red blood cells through the production of hydrogen peroxide (H2O2). The production of H2O2 by the bacterial infection can also cause damage to DNA, and kill cells within the lungs. Pneumococcal pneumonia causes fever and chills, coughs, difficulty breathing, and chest pain. If the infection spreads to the brain and spinal cord, it can cause pneumococcal meningitis, characterised by a stiff neck, fever, confusion, and headaches.

Pneumococcal infection is responsible for 1-2 million infant deaths worldwide, every year. During influenza epidemics, S. pneumoniae is associated with higher mortality in patients infected with both microorganisms. It is thought that S. pneumoniae and Haemophilius influenzae have a synergistic effect on one another, when infecting the same host.

Risk Factors

The primary risk factors for infection are:

1. Age. People younger than 2 or older than 65 years old are at highest risk of infection
2. Absence of normal spleen function (asplenia / hyposplenia)
3. Alcoholism
4. Diabetes mellitus
5. Influenza
6. Defects in specific aspects of the immune system, such as the complement cascade or antibodies
7. HIV infection
8. Strains of S. pneumoniae that are particularly virulent

Diagnosis

S. pneumoniae can be readily identified by culturing bacterial samples on blood agar. Due to the hemolytic properties of the bacteria, they will turn blood agar to dark green. However, growing bacterial cultures can take time. Therefore, it is more efficient to diagnose an infection using more modern techniques.

The first technique involves the detection of parts of streptococcus bacterium, known as antigens, in urine. C polysaccharide, a main component of the bacterial cell wall, can be detected in a number of bodily fluids. Immunochromatography is used to test for the presence of specific antigens, and compares favorably to diagnosis via bacterial culture.

The second technique involves an assay based on detection of S. pneumoniae-specific DNA sequences. These can be performed extremely quickly, and the high specificity conferred by using DNA as a target minimizes the risk of error.

Treatment

Antibiotics are the first line of treatment for bacterial infections, and are effective against pneumococcus. However, treatment of pneumococcal infections is dependent on the strain that a patient is infected with. Some strains of S. pneumoniae have acquired a resistance to specific antibiotics, and therefore cannot be reliably treated with them. During diagnosis, cases of S. pneumoniae will often be tested for susceptibility to antibiotics, and the physician will make a judgement of treatment course based on the findings.

Prevention is considered to be better than a cure, and therefore vaccination is preferable to having to treat a disease. Two vaccines for pneumococcal infection are available, and both work on the same mechanism: delivery of antigens to provoke an immune response in the recipient. However, since the introduction of these vaccines to combat the spread of S. pneumoniae, some strains have been observed that do not carry the antigens that the body has been sensitized to. Other vaccine approaches are being considered, looking for antigens that are more readily conserved between bacterial strains.

References

• CDC website. http://www.cdc.gov/pneumococcal/ Accessed 01-Aug-16
• Lysenko ES, Ratner AJ, Nelson AL, Weisner JN. The Role of Innate Immune Responses in the Outcome of Interspecies Competition for Colonization of Mucosal Surfaces. PLoS Pathogens 2005; 1 (1): e1
• Rai P, Parrish M, Tay IJJ, et al. Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells. Proc Natl AcadSci 2015; E3421 – E3430
• Tikhomirova A, Kidd SP. Haemophilus influenzae and Streptococcus pneumoniae: living together in a biofilm. Pathogens and Disease 2013; 69: 114 – 126
• Van der Poll T, Opal SM. Pathogenesis, treatment, and prevention of pneumococcal pneumonia. Lancet 2009; 374 (9700): 1543 – 1556

Written by

Oliver Fleck

Oliver graduated from Imperial College London, after doing a research-based Masters in Bioengineering. He is currently a Ph.D student at Cambridge University, where his research involves lots of fiddly work with cells. His spare time is spent climbing, cycling, riding motorbikes, and making games using 3D printing and laser cutting.